Figure 3.

Quantitation of isotopic distributions from yeast lysate. (A) Overall quantitation of the distributions (after filtering for a minimum signal/noise of 15) in yeast lysates that were mixed in ratios of 1:1, 3:1, and 5:1 (K₆₀₂:K₀₈₀). Measured (box and whiskers) and true (dotted lines) ratios for all three samples is displayed with the median (stripe), mean (square), interquartile range (25th to 75th, box), and 1.5X interquartile range (whiskers). (B) Quantitation of the +12 charge state of ubiquitin (Uniprot P0CG63) from the same mixtures, demonstrating ratios of 0.77, 2.09, and 4.27, in good agreement with the mixing ratios of 1:1, 3:1, and 5:1.