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Response of the method to LNT. (a) The manufacturer default conditions for peptides produced a large amount of unintended LNT fragments (m/z 366) in the MS scan mode. (b) After the instrument optimization, the fragment ion signal was diminished and the quasimolecular ion was increased: blue squares, GlcNAc (N-acetylglucosamine); blue circles, Glc (glucose); yellow circles, Gal (galactose).
