The fluorescence of coralyne is proportional to the concentration
of c-di-AMP. Condition: [Coralyne] = 10 μM, [c-di-AMP] = 0,
5, 10, 20, and 40 μM; buffer: 50 mM Tris-H3PO4 (pH 9.2) containing 250 mM KBr, showing c-di-AMP concentration
dependence on fluorescence enhancement. Temperature = 10 °C.
ex. 420 nm, em. 475 nm.