. 2014 Feb 4;86(5):2412–2420. doi: 10.1021/ac403203x

Table 1. Fluorescence Quenching of Coralyne with KBr^a.

	in absence of c-di-AMP						in presence of 40 μM of c-di-AMP
quencher KBr [mM]	τ_I (νσ)	f_i	τ_αω̅γ (νσ)	χ²	τ_ο/τ^b (I₀/I)	I	τ_I (νσ)	f_i	τ_αω̅γ (νσ)	χ²	τ_ο/τ^b (I₀/I)	I
0	14.22	1	14.2	1.85	1	242						n/a
	9.82	0.317			(1)		n/a	n/a	n/a	n/a	n/a
	<30>	0.683	24.97	1.16

10	11.61	1	11.61	1.42	1.22	96	12.13	1	12.13	30		428
	9.83	0.592			(2.38)		2.33	0.137
	<30>	0.408	18.06	1.21			19	0.863	16.73	1.86	1
							1.29	0.072
							5.92	0.131
							28.73	0.797	23.76	1.04

25	9.86	1	9.86	1.27	1.44	48	12.39	1	12.04	31		411
	8.86	0.731			(4.75)		2.5	0.136
	<30>	0.269	14.55	1.10			19.43	0.864	17.11	1.4	0.98
							1.46	0.075			(1.04)
							6.79	0.144
							30.52	0.781	24.52	1.21

50	8.52	1	8.52	1.10	1.67	14.3	12.04	1	12.04	25		420
	3.50	0.063			(15.94)		2.31	0.139
	8.99	0.937	8.64	1.04			18.58	0.861	16.32	1.54	1.02
							1.48	0.073			(1.02)
							7.04	0.148
							31,3	0.779	25.52	1.21

75	7.11	1	7.11	1.37	2.0	12.3	11.9	1	11.9	26		386
	4.11	0.228			(18.54)		2.49	0.141
	8.22	0.772	7.29	1.12			18.63	0.859	16.35	1.6	1.02
							1.4	0.064			(1.11)
							6.82	0.154
							33.67	0.782	27.46	1.14

100	6.67	1	6.67	1.35	2.13	6.6	11.89	1	11.89	27		387
	2.18	0.131			(34.55)		2.5	0.142
	7.19	0.869	6.53	1.03			18.7	0.858	16.4	1.51	1.02
							1.37	0.067			(1.11)
							6.33	0.149
							30.39	0.784	24.86	1.06

250	4.38	1	4.38	3.77	3.25	3.9	11.36	1	1	28		317
	6.19	0.506			(58.46)		2.37	0.152
	2.02	0.494	4.13	1.03			16.96	0.848	14.75	1.69	1.13
							1.07	0.088			(1.35)
							5.14	0.153
							23.05	0.759	18.37	1.09

Ex. 445 nm, Em 480/30 nm. Condition: [coralyne] = 10 μM, [c-di-AMP] = 40 μM; buffer: 50 mM Tris-H₃PO₄ (pH 7.5) at 25 °C; average lifetime τ_avg = Σ f_iτ_i.

For relative lifetime, single lifetime fit was used for KBr and average lifetimes from double-exponential fit in the presence of c-di-AMP.

Table 1. Fluorescence Quenching of Coralyne with KBra.

Table 1. Fluorescence Quenching of Coralyne with KBr^a.