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. 2014 Apr 10;10(4):e1004253. doi: 10.1371/journal.pgen.1004253

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(A) B. bacteriovorus ΔmglA HI cells showed gliding motility on 1% agarose/CaHEPES. Gliding was sustained and progressive (cells were not hyper-reversing), as in the arrowed cell which moved at 9.49 µm hr⁻¹. Each panel is a 15 minute timepoint, starting from 540 minutes after the cells were added to the agarose surface (three “bystander” cells that have not yet commenced gliding represent a stationary marker). (B) B. bacteriovorus MglA-His₈ HD cells showed a high incidence of reversals during gliding motility on 1% agarose/CaHEPES compared to wild-type cells. Each larger panel shows a “trail-montage” of 60 minutes of gliding motility (150 second per frame): MglA-His₈ cells show no progressive gliding motility (reversing rapidly), whilst wild-type cells show sustained runs of gliding (seen as curving trails with direction changes). (C) Smaller panels show individual wild-type and MglA-His₈ HD cells gliding from an original start point (indicated by white dashed region), starting at 180 minutes after addition to the agarose surface and at 150 second intervals; arrow indicates direction of movement.