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. 2014 Apr 10;10(4):e1004249. doi: 10.1371/journal.pgen.1004249

Figure 4. RT-PCR analysis of alternatively spliced products under stress conditions.

Figure 4

A. Analysis of RPL22B in various stress conditions. Shown are the RT-PCR products obtained from the wild-type or upf1Δ mutant strain after growth in the following conditions: SDC, synthetic define complete medium at 30°C; -AA, 10 minutes in SDC medium at 30°C lacking amino acid (-AA); 25°C, log phase at 25°C in YPD; H.S, 20 minutes at 42°C in YPD; YPD: log phase at 30°C in YPD; LiCl, incubation with 300 mM LiCl in YPD at 30°C for 10 minutes; RAP control, see Materials and Methods; RAP, treatment with Rapamycin for 20 minutes. B. RT-PCR analysis of GCR1 alternative splicing in heat-shock conditions. Labeling of the different species is similar to that of Figures 2 and 3.