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. 2014 Apr 10;10(4):e1004202. doi: 10.1371/journal.pgen.1004202

Figure 7. Activation of heterochromatic hsp82-2001 occurs in the presence of minimal H2A.Z levels and is unimpaired by deletion of HTZ1.

Figure 7

(a) ChIP analysis of H2A.Z at the indicated hsp82 alleles under NHS (−) and 20 min HS (+) conditions conducted as in Figure 4B, with H2A.Z content normalized per nucleosome (Myc-H4 abundance). Shown are means ± S.D. (N = 2). (b) hsp82-2001 transcript abundance was determined in WT and htz1Δ cells (sir4Δ and SIR+ as indicated) subjected to heat shock for the indicated periods of time. RNA was isolated, cDNA synthesized and HSP82 transcripts were quantified using RT-qPCR and normalized to SCR1 as in Figure 4A. Depicted are means ± S.D. (N = 2; qPCR = 4).