Figure 2. Subcellular localization of Ecd and the U5 snRNP proteins.

(A) Brr2 was enriched in cell nuclei while Ecd, Prp8, Snu114, and Aar2 were detected in the cytoplasm. The Flag and Myc epitope-tagged proteins were expressed in S2 cells; mRFP::Prp8 and GFP::Ecd (bottom row) were co-transfected. (B) Inhibition of nuclear export with leptomycin B resulted in nuclear retention of the GFP::Ecd protein. Nuclei are stained with DAPI. All images are single confocal sections. Scale bars, 5 µm.