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. 2014 Apr 10;10(4):e1004287. doi: 10.1371/journal.pgen.1004287

Figure 3. Ecd is required for survival of imaginal disc cells.

Figure 3

(A–F) In contrast to abundant, sizable control clones (green in A and GFP-negative in E, yellow arrowheads), ecdl(3)23 homozygous mutant clones could be recovered neither in eye/antennal (B) nor wing (F) imaginal discs. Simultaneous inhibition of apoptosis with a pan-caspase inhibitor p35 (ecdl(3) 23 p35) (C) or cell growth enhancement with activated RasV12 (ecdl(3) 23 rasV12) (D) yielded rare and extremely small clones in eye/antennal discs (white arrowheads). Fasciclin III (FasIII) staining of lateral cell membranes reveals the overall tissue morphology. (G–H) ecdRNAi clones (green) were eliminated from the wing imaginal epithelium within 72 h after induction using the heat-shock flip-out technique. Only rare ecdRNAi cells labeled phospho-histone 3 (pH3) positive relative to many mitotically active surrounding control cells. Scale bars, 50 µm.