Figure 4. ADARs-mediated enzymatic reactions is associated with the macaque editome.

(A) The enriched (above the top line) and depleted (below the bottom line) nucleotides nearby the focal editing sites are displayed in Two-Sample Logo, with the level of preference/depletion shown in height proportional to the scale. (B) The editing sites were divided into four categories on the basis of the local sequence context nearby the editing site, as described in Materials and Methods . For each category, levels of RNA editing are shown in boxplots according to the tissue types. (C) Distribution of the percentages of editing sites showing tissue distribution of editing levels positively correlated with the expression of ADARs (Spearman's rank correlation coefficient at ≥0.5), for 10,000 permutation datasets neglecting tissue relationships for the tissue expression profile. The percentage for the real data was indicated by the arrow with Monte Carlo p-value. (D) Distributions of R² values in models assuming association of editing level with ADARs expression are shown as the Real Data, as well as the Background, which correspond to randomly shuffled profiles. (E, F) The tissue expression profiles of ADAR1 or ADAR2 were ordered based on RNA expression levels, and normalized editing levels of A-to-G sites were aligned accordingly. These A-to-G editing sites showed similar trends in the distribution of editing levels along the ordered tissue expression profile of ADAR1 (E) or ADAR2 (F).