Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 10;10(4):e1004045. doi: 10.1371/journal.ppat.1004045

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Robinson et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Differentiated NPSCs transduced with adeno-LC3-GFP were infected with dsRED-CVB3 (moi = 0.1) and observed by fluorescence microscopy up to 3 days PI. (A) Transduced NPSCs (green) and viral protein expression (red) were readily observed by day 1 PI. (B) Differentiated NPSCs infected with virus showed signs of cellular blebbing by day 2 PI (white arrow). (C–D) Abundant shed EMVs were readily observed by day 3 PI. EMVs (white arrows) contained viral protein (red) and expressed a marker for autophagosomes (LC3-GFP, green). (E–F) Higher magnification of (C) and (D) showed colocalization of viral protein and LC3-GFP in shed EMVs.