Skip to main content
. Author manuscript; available in PMC: 2014 Apr 10.
Published in final edited form as: Nat Med. 2013 Jun 30;19(8):1061–1066. doi: 10.1038/nm.3192

Figure 4. Lrh-1 is critical for decidualization of both the mouse and human endometrial stroma.

Figure 4

(a) Oil-induced deciduoma formation at dpc 8 in pseudopregnant CON mice, absent in both cKO and reduced in cKO+P4 mice, scale bars, 10 mm (b) Ratio of oil-treated to control uterine horn weights (Mean ± SEM) at dpc 8 following decidual stimulus at dpc 4 (c–d) Histological comparison of placental sections at dpc 12 from CON (c) and cKO+P4 (d) mice. (La, placental labyrinth; Sp, spongy zone; St, maternal stroma; M, myometrium). Scale bars, 100 μm. (e) Macroscopic comparison of placentas dissected from CON and cKO+P4 mice at dpc 17, scale bar, 10 mm. (f) Nuclear localization of Lrh-1 in the proliferative and secretory human endometrium, scale bar 10 μm (g) Abundance of transcripts for LRH-1(NR5A2) and the decidual markers PRL and IGFBP1 in human endometrium during the proliferative and secretory phases of the menstrual cycle. MCF-7 breast cancer cells were used as a positive control for LRH-1 transcript expression. (h) Quantitative PCR analysis demonstrating knockdown of Lrh-1 transcripts by siRNA (initiated on Day 0) in primary cultures of human endometrial stromal cells undergoing decidualization in vitro; and abundance of decidual marker genes LRH-1 (NR5A2), PRL, WNT4 and IGFBP1 in human stromal cells in vitro. Asterisks indicate significant differences (P<0.05) between control and siRNA knockdown at each sampling time.