Figure 3. The role of CCP domain-containing proteins in flaviviral infection.

Each CCP gene was silenced through thoracic microinjection of dsRNA and the effect on the viral burden was assessed on 6 days post-DENV-2 (A) or YFV (B) infection. GFP dsRNA-inoculated mosquitoes served as mock controls. The viral loads were measured via qPCR, and normalized against A. aegypti actin. One dot represents 1 mosquito and the horizontal line represents the median. The data were analyzed statistically using the non-parametric Mann-Whitney test. The results were combined from 2 independent experiments.