Figure 8. Immunofluorescence cell imaging of PV1-infected HeLa cells in the presence and absence of BSO.

(A) HeLa cells were infected with PV1-wt or PV1-BSOr (VP3 Q178L) at a moi of 10 at 37°C. After 4 hours incubation, the infected cells were probed for mature virus with A12 primary antibody (anti-PV human serum), which binds specifically to mature virus and provirions (Nihal Altan Bonnet, unpublished results), and Alexfluor 488-conjugated secondary antibody (green color). The localization of 3AB, a member of the replication complex, was determined in the same cell using 3AB mouse monoclonal antibody and Alexfluor 555-conjugated secondary antibody (red color). The scale is 5 µm. (B) Infected cells were probed for capsid precursors using VP3 polyclonal antibody and Alexfluor 488-conjugated secondary antibody (green color). The localization of 2C^ATPase, another nonstructural protein on the replication complex, was determined in the same cell using 2C mouse monoclonal antibody and Alexfluor 555-conjugated secondary antibody (red color).