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. 2014 Apr 10;9(4):e93758. doi: 10.1371/journal.pone.0093758

Figure 2. Standard curves for Ct value against RNA copy number for Seg-1 and Seg-3 RT-PCR.

Figure 2

Standard curves generated by plotting Ct values against corresponding log copy number of viral dsRNA (RSArah01/03) in Seg-1 and Seg-3 assays. Each dilution was tested in quadruplicate. Non-linearity of the lowest and highest dilutions were excluded from the analysis.