Table 2. List of primers and probes for AHSV virus-species- and type-specific assays.
| Assay Type | Oligo Name | Oligo Sequence (5′-3′) |
| Seg-1 based/virus-species-specific | ||
| AHSV/Seg-1/FP/1310-1329 | CCTGAAGATATGTATACATC | |
| AHSV/Seg-1/RP/1444-1461 | ACTAACGCTTTAATCCGC | |
| AHSV/Seg-1/P/1400-1423 | CCCAACGCRAARGGTGGACGTGG | |
| Seg-3 based/virus-species-specific | ||
| AHSV/Seg-3/FP/2030-2050 | AGATATYGTAAGGTGGAGTCA | |
| AHSV/Seg-3/RP/2115-2136 | CTAACATCAARTCTTCAAARTC | |
| AHSV/Seg-3/P/2085-2107 | ATCGCCCAAGCTTCCCTATTCAA# | |
| Seg-2 based/type-specific | ||
| AHSV/SRT-1/FP/1143-1163 | GAATGGTAAGCTTTGGATTGA | |
| AHSV/SRT-1/RP/1225-1207 | TTAGAGGCGCTCGGTTCTC | |
| AHSV/SRT-1/P/1165-1187 | CATAAACAAACGGTGAGTGAGCA | |
| AHSV/SRT-2/FP/1763-1783 | AGTGGACTTCGATTATAGATG | |
| AHSV/SRT-2/RP/1897-1878 | CTGTCTGAGCGTTAACCTTC | |
| AHSV/SRT-2/P/1863-1842 | TTCAACCGTCTCTCCGCCTCTC# | |
| AHSV/SRT-3/FP/809-829 | TAGAAAGAATGATGAGCAGTG | |
| AHSV/SRT-3/RP/901-921 | TAATGGAATGTCGCCTGTCTT | |
| AHSV/SRT-3/P/845-870 | CCGTTATTGAGAGCGTCATAAGATTC | |
| AHSV/SRT-4/FP/1934-1956 | GTTTCGTATCATATTGGTATAGA | |
| AHSV/SRT-4/RP/2079-2101 | GATATATATATGTGGATGCATGG | |
| AHSV/SRT-4/P/1996-2020 | CYGGAYATGAATGAGAAACAGAAGC | |
| AHSV/SRT-5/FP/1875-1895 | GAGACACATCAAGGTTAAAGG | |
| AHSV/SRT-5/RP/2035-2014 | CAGGATCAAACTGTGTATACTT | |
| AHSV/SRT-5/P/1964-1985 | TTGAAGCAAGGAATCTATTGACTTT | |
| AHSV/SRT-6/FP/1285-1304 | GTTGGTCATTGGGTCGATTG | |
| AHSV/SRT-6/RP/1505-1485 | GACGAATTTGATCCCTTCTTG | |
| AHSV/SRT-6/P/1385-1408 | TGATGTCGGGTATGAACAAACTGG | |
| AHSV/SRT-7/FP/1159-1179 | TGGATCGAGCATAAGAAGAAG | |
| AHSV/SRT-7/RP/1282-1302 | CCAATCAACCCARTGTGTAAC | |
| AHSV/SRT-7/P/1213-1237 | ACCAAAATCGTCCGATGCTAGTGC | |
| AHSV/SRT-8/FP/2122-2141 | GGTGAGCGGATTGTTGATAG | |
| AHSV/SRT-8T/RP/2293-2274 | CTGAATCCACTCTATACCTC | |
| AHSV/SRT-8/P/2169-2146 | CGACAAATCATACCACACGATCTC# | |
| AHSV/SRT-9/FP/1931-1952 | TATCATATTGGTATCGAGTTCG | |
| AHSV/SRT-9/RP/2074-2054 | AAGTTGATGCGTGAATACCGA | |
| AHSV/SRT-9/P/1978-2000 | ACATCCTCAATCGAYCTCCTCTC# | |
The names of the oligonucleotide primers and probes indicate the group- or type-specificity of the assay. Seg-1 and Seg-3 indicate the group-specific assays while SRT-1 to SRT-9 indicate respective type-specific assays. FP, RP or P stand for ‘forward primer’, ‘reverse primer’ and ‘probe’ respectively. The numbers represent annealing positions on the genome segment. Probes indicated (#) are complementary to the negative strand.