Table 5. Specificity of AHSV virus-species-specific assays (Seg-1 and Seg-3).
| Virus species-serotype | Origin | ORC reference number | Ct values | |
| Seg-1 | Seg-3 | |||
| Reference strain AHSV-1 | RSA | RSArah1/03 | 17.36 | 19.13 |
| Reference strain AHSV-2 | RSA | RSArah2/03 | 16.56 | 16.02 |
| Reference strain AHSV-3 | RSA | RSArah3/03 | 21.1 | 22.02 |
| Reference strain AHSV-4 | RSA | SPArah4/03 | 14.37 | 16.09 |
| Reference strain AHSV-5 | RSA | RSArah5/03 | 19.02 | 20.52 |
| Reference strain AHSV-6 | RSA | RSArah6/03 | 20.00 | 19.81 |
| Reference strain AHSV-7 | Kenya | KENrah7/03 | 17.22 | 16.17 |
| Reference strain AHSV-8 | RSA | RSArah8/03 | 17.08 | 16.41 |
| Reference strain AHSV-9 | Pakistan | PAKrah9/03 | 16.73 | 12.63 |
| EEV-1 | RSA | RSA1967/03 | No Ct | No Ct |
| EEV-2 | RSA | RSA1971/06 | No Ct | No Ct |
| EEV-3 | RSA | RSA1974/06 | No Ct | No Ct |
| EEV-4 | RSA | RSA1976/03 | No Ct | No Ct |
| EEV-5 | RSA | RSA1976/06 | No Ct | No Ct |
| EEV-6 | RSA | RSA1991/03 | No Ct | No Ct |
| PHSV | Peru | PER1997/01 | No Ct | No Ct |
| EHDV-1 | USA | USA2001/03 | No Ct | No Ct |
| EHDV-5 | Australia | AUS1977/01 | No Ct | No Ct |
| EHDV-7 | Australia | AUS1981/06 | No Ct | No Ct |
| EHDV-7 | Israel | ISR2006/01 | No Ct | No Ct |
| BTV-16 | Pakistan | RSArrrr/16 | No Ct | No Ct |
| BTV-2 | India | IND1982/01 | No Ct | No Ct |
| BTV-9 | Turkey | TUR1998/04 | No Ct | No Ct |
| BTV-8 | UK | UKG2007/64 | No Ct | No Ct |
| BTV-1 | RSA | RSArrrr/01 | No Ct | No Ct |
| BTV-12 | RSA | RSArrrr/12 | No Ct | No Ct |
| C. sonorensis | No Ct | No Ct | ||
| Uninfected BHK cells | No Ct | No Ct | ||
| Uninfected Vero cells | No Ct | No Ct | ||
| Uninfected KC cells | No Ct | No Ct | ||
| Uninfected horse blood | No Ct | No Ct | ||
Representatives of different serotypes and topotypes from five different Orbivirus species (AHSV, EEV, PHSV, EHDV and BTV) were tested to confirm the specificity of assays for AHSV dsRNA. Further details on these isolates can be obtained from ORC http://www.reoviridae.org/dsRNA_virus_proteins/ReoID/viruses-at-iah.htm [61]. EEV = Equine encephalosis virus; PHSV = Peruvian horse sickness virus; EHDV = Epizootic haemorrhagic disease virus; BTV = Bluetongue virus. RSA = Republic of South Africa.