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. 2014 Apr 10;9(4):e93836. doi: 10.1371/journal.pone.0093836

Figure 2. Electrical properties of Lim1 and Isl1-positive LMC neurons.

Figure 2

A) Overall electrical activity profiles of Isl1+ and Lim1+ LMC subpopulations at HH St. 29/30 are similar; traces shown are recorded from two different preparations. B) Neurons filled with Alexa 568 dye are identified by post-recording immunostaining with Lhx1, Isl1 and Foxp1 antibodies. C) Comparison of membrane potential and membrane resistance (D) reveals both of these characteristics to be similar between cell types, whereas the membrane capacitance is higher in Isl1+ neurons (E). The magnitude of individual cycles is no different between cell types (F), though cycles are longer in Lim1+ cells (G) and there is a shorter interval between cycles in Lim1+ cells (H). The average number of cycles in an episode is not different (I), nor are episodes (J) or the intervals between episodes (K) longer in one cell type than another. Camera lucida tracing of filled neurons (L) allows counts of primary (black, M) and secondary (grey, N) processes, demonstrating no difference in branch characteristics of Isl1+ and Lim1+ neurons at the stages examined.