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. 2014 Apr 10;9(4):e93983. doi: 10.1371/journal.pone.0093983

Figure 3. Increased PHKA1 exon 19 inclusion in DM2 patients.

Figure 3

A) EAA analysis identified an AS event on exon 19 of PHKA1 mRNA transcript ENST00000339490. The AS area is enlarged and exon 19, more frequently included in DM2 patients, is highlighted in solid gray. B) The box plot shows the increased expression of Affymetrix probe set 4012322 recognizing exon 19, in DM2 patients compared to CTR (n = 10, **** p<0.0001). Values are normalized for the levels of the whole transcript. The splice index (SI) is indicated. C) Validation qPCR assays were performed using the specific primer pair indicated as black arrowhead in panel A. Results are shown as fold change (DM2 = 19, CTR = 15; ** p<0.01).