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. 2014 Apr 10;9(4):e93983. doi: 10.1371/journal.pone.0093983

Figure 6. Increased CAMK2G exon 18 inclusion in DM2 patients.

Figure 6

A) EAA analysis identified an AS event on exon 18 of CAMK2G mRNA transcript ENST00000394763. The AS area is enlarged and exon 18, more frequently included in DM2 patients, is highlighted in solid gray. B) The box plot shows the increased expression of Affymetrix probe set 3294875 recognizing exon 18, in DM2 patients compared to CTR (n = 10, **** p<0.0001). Values are normalized for the levels of the whole transcript. The splice index (SI) is indicated. C) Validation qPCR assays were performed using the specific primer pair indicated as black arrowhead in panel A. Results are shown as fold change (DM2 = 19, CTR = 15; **** p<0.0001). D) Reverse transcription PCR products obtained with the specific primer pair indicated as black lines in panel A, were resolved on 5% polyacrylamide electrophoresis gel. The 250 bp fragment containing exon 18 was more abundant in DM2 patients.