Table 3. Determination of glycosidic linkages in the EPOLs of B. ambifaria before (Ba) and after carboxyl reduction (Ba-R), and B. multivorans C1576 separated on SP2330 (Bm-I) and on HP1 (Bm-II) columns.
Linked residuea | RRTb | Ba | Ba-R | Bm-I | Bm-II | RRTc |
2-Rha | 0.91 | 0.72 | 0.64 | 1.00 | 1.00 | 0.84 |
3-Rha | 0.93 | 0.43 | 0.43 | 0.86 | ||
t-Gal | 1.00 | 2.27 | 2.11 | 1.71 | 1.82 | 1.00 |
3-Glc | 1.13 | 1.00 | 1.00 | 0.68 | ||
2-Man+3-Man | 1.14 | 0.92 | ||||
3-Glc+2-Man | 1.31 | 1.17 | ||||
3-Man | 0.47 | 1.20 | ||||
3-Gal | 1.17 | 0.20 | 0.21 | 0.13 | 0.17 | 1.22 |
6-Gal | 1.30 | 0.20 | 0.19 | |||
2,4-GlcD2 d | 1.40 | 0.09 | ||||
3,6-Man | 1.43 | 0.39 | 0.50 | 0.30 | 0.29 | 1.51 |
2,3,4-GlcD2 d | 1.47 | - | 0.21 | - | - |
Position of glycosidic linkages.
Relative retention time on SP2330 column.
Relative retention time on HP1 column.
C6 di-deuterated according to GC–MS.