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. 2014 Apr 10;9(4):e94372. doi: 10.1371/journal.pone.0094372

Table 3. Determination of glycosidic linkages in the EPOLs of B. ambifaria before (Ba) and after carboxyl reduction (Ba-R), and B. multivorans C1576 separated on SP2330 (Bm-I) and on HP1 (Bm-II) columns.

Linked residuea RRTb Ba Ba-R Bm-I Bm-II RRTc
2-Rha 0.91 0.72 0.64 1.00 1.00 0.84
3-Rha 0.93 0.43 0.43 0.86
t-Gal 1.00 2.27 2.11 1.71 1.82 1.00
3-Glc 1.13 1.00 1.00 0.68
2-Man+3-Man 1.14 0.92
3-Glc+2-Man 1.31 1.17
3-Man 0.47 1.20
3-Gal 1.17 0.20 0.21 0.13 0.17 1.22
6-Gal 1.30 0.20 0.19
2,4-GlcD2 d 1.40 0.09
3,6-Man 1.43 0.39 0.50 0.30 0.29 1.51
2,3,4-GlcD2 d 1.47 - 0.21 - -
a

Position of glycosidic linkages.

b

Relative retention time on SP2330 column.

c

Relative retention time on HP1 column.

d

C6 di-deuterated according to GC–MS.