Table 3. Characterization of 15 polymorphic microsatellite loci in Portunus trituberculatus.
| Transcript IDs | (Type) No. | Primer sequence (5′-3′) | Length | Ta | Na | Ho | He | PIC |
| comp57884_c0 | (TG)9 | F: TTGGAAGTTTTGTTGGCGGC | 199 | 59 | 3 | 0.59 | 0.64 | 0.56 |
| R: ACGTTGTGGTCACTTGTTGC | ||||||||
| comp32503_c0 | (TG)9 | F: GCGGCGTGGTAATCTCTATGT | 199 | 60 | 4 | 1 | 0.74 | 0.70 |
| R: CTAACAAGACATCCACGCACG | ||||||||
| comp333871_c0 | (TG)8 | F: GCAGGGGCTTCTTCACCTTA | 235 | 60 | 5 | 0.96 | 0.79 | 0.74 |
| R: CCCCTCCTTCCAATTTGTACCT | ||||||||
| comp31578_c0 | (CA)8 | F: GACCAACAGGCGACCGAG | 134 | 57 | 2 | 0.2 | 0.18 | 0.16 |
| R: AGCCGCTTCCCGAGATTC | ||||||||
| comp49316_c0 | (TC)9 | F: TGCGTTGCGTAACTGAGTCT | 269 | 59 | 4 | 1 | 0.63 | 0.56 |
| R: CTTGTCAGTGCTTCCTTGCC | ||||||||
| comp55771_c0 | (TG)10 | F: AAGACCCCGAGGAAGAGC | 199 | 55 | 5 | 1 | 0.75 | 0.69 |
| R: GCAAGGCATATCCAACAT | ||||||||
| comp54398_c4 | (TC)9 | F: CTTTGTGTGTTGGGTTGGGC | 142 | 58 | 3 | 0.72 | 0.55 | 0.45 |
| R: AACAATGCCCACAACTCAGG | ||||||||
| comp17478_c0 | (GT)10 | F: GCATAGAACGAGTGATACTAGATGC | 235 | 59 | 8 | 0.92 | 0.79 | 0.78 |
| R: CACACACACACACACACACA | ||||||||
| comp53876_c0 | (GT)10 | F: ACAGCGTCAGGAAGCAATCA | 209 | 60 | 1 | 0 | 0 | 0 |
| R: ATTGCCTCCTTCCCAATGCA | ||||||||
| comp49119_c1 | (CA)9 | F: TGGTTTTGCCACTCCACACT | 182 | 59 | n/a | |||
| R: TGTCAGCCACGACACTACTT | ||||||||
| comp44386_c0 | (CA)7 | F: TTGCGTGTGGAGGAAGTGTT | 277 | 60 | n/a | |||
| R: GAGAGGTGGATGGGGAGACT | ||||||||
| comp16699_c0 | (AG)7 | F: TTCTCATTCTTGGCTCGCGT | 205 | 60 | n/a | |||
| R: TCACATGCTCCGAGGATTGG | ||||||||
| comp24638_c0 | (AG)8 | F: CGTGTGTGCGTGTTTGTCTT | 279 | 58 | n/a | |||
| R: AGTTTTCCTCCTTTACGCATCA | ||||||||
| comp58479_c1 | (TG)6 | F: AGGACCATAACAAGGCCACG | 176 | 60 | n/a | |||
| R: CTGCAACACAGCACTGACAG | ||||||||
| comp1580_c0 | (TG)7 | F:CACCCAAGCTCTCTTCCTGG | 228 | 59 | n/a | |||
| R: ACCAACAGACAGGGAGAGGA |
Ho observed heterozygosity, He expected heterozygosity, Na observed number of alleles, Ta annealing temperature, PIC polymorphic information content, n/a indicates that no PCR amplification.