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. 2014 Feb 15;15(3):908–914. doi: 10.1021/bm4017594

Figure 1.

Figure 1

Fluorescence spectra of 1,8-ANS (80 μM) with 0.5 mg/mL SE8Y, S2E8Y, and S4E8Y in phosphate buffer saline at 25 °C. Fluorescence spectra of SE8Y and SE8Y in 8 M urea solution were included for controls. The inset shows the SDS-PAGE gel analysis of purified SE8Y, S2E8Y, and S4E8Y proteins.