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Flow cytometry analysis of HeLa cells treated with PBS (a) and S1-Dox nanoparticles at 37 °C (b) and 4 °C (c). The weaker doxorubicin fluorescence observed at 4 °C compared to that at 37°, indicating a decreased cellular uptake of S1-Dox nanoparticles at a lower temperature and suggesting the uptake of S1-Dox is an energy-dependent process.
