Table 1. Correlation between expected results and automated extraction method performed as quality control for EBV DNA load measurement.
Expected results | EBV DNA load | |
Log copies | Log copies | Ct mean |
2 | 1.94 | 30.63 |
3 | 2.90 | 27.48 |
4 | 3.96 | 24.00 |
5 | 4.97 | 20.69 |
The table lists Ct value and log copies/reaction using standard commercial EBV calibrators (EBV Q-PCR Alert Standard kit, ELITech Group, Puteaux, France). Negative cell-free CSF samples were spiked with EBV DNA concentrations ranging from 105 to 102 copies/microl.