Table 1.
Guest | CD-alginate with controls | CD alginate with naphthyl-RGDS | CD-alginate with ada-RGDS | Dynamics: CD alginate with 10 μM naphthyl-RGDS. After 3 hr addition of 10 μM ada-RGES | ||||
---|---|---|---|---|---|---|---|---|
without guest | 10 μM acetyl-RGDS | 10 μM ada-RGES | 10 μM | 100 μM | 10 μM | 100 μM | ||
Projected area (μm2) | 795 ± 33 | 745 ± 46 (n.s.) | 732 ± 34 (n.s.) | 1260 ± 37 (***) | 835 ± 49 (n.s.) | 1587 ± 51 (***) | 1444 ± 53 (***) | 709 ± 28 (n.s.) |
Convex hull (μm2) | 1293 ± 57 | 1276 ± 81 (n.s.) | 1436 ± 63 (n.s.) | 2001 ± 69 (***) | 1429 ± 92 (n.s.) | 2565 ± 85 (***) | 2402 ± 90 (***) | 1123 ± 56 (n.s.) |
Circularity (a.u.) | 0.35 ± 0.01 | 0.32 ± 0.01 (n.s.) | 0.34 ± 0.01 (n.s.) | 0.30 ± 0.01 (*) | 0.28 ± 0.01 (***) | 0.28 ± 0.01 (***) | 0.25 ± 0.01 (***) | 0.35 ± 0.01 (n.s.) |
Stress fibers observed (%) | 8.4 | 4.8 | 12 | 33 | 6.2 | 48 | 43 | 12 |
Statistical significance given compares samples to the “without guest” column. Noted errors correspond to standard errors.
statistical significance with P < 0.001
**: P < 0.01
P < 0.05
n.s.: P > 0.05