Skip to main content
. 2014 Apr 10;9(4):e94413. doi: 10.1371/journal.pone.0094413

Figure 4. Amino acids 508–713 of Axin are required for colocalisation with truncated APCL.

Figure 4

DLD1 cells were transiently transfected on day 1 with wild-type or mutant N-terminal myc-tagged Axin, either individually or in combination with the yAPCL1179 expression vector. The cells were fixed on day 3 and were stained with an anti-myc antibody and Hoechst dye. The presence of amino acids 508–713 in the myc-Axin deletion mutants resulted in colocalisation with yAPCL1179. Where applicable, the percentages indicate the proportion of different localisation patterns in the transfected cells. The imaging parameters were identical for each type of tag. Bar, 10 μM.