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. 2014 Apr 10;9(4):e94548. doi: 10.1371/journal.pone.0094548

Figure 1. hAAT has protective and immunoregulation dual effects analysed by CTL assay.

Figure 1

The effects of hAAT on a β islet cell line were examined via an extended CTL assay in vitro (n = 3). BALB/c mice were immunized twice with NIT-1 or NIT-vector or NIT–hAAT cells, and the splenocytes were collected. These splenoctyes were used as responder cells in a 7 day MLR, while NIT-1 or NIT-vector or NIT–hAAT cells pretreated with mitomycin C were used as stimulator cells. An in vitro CTL assay was later performed using NIT-1 or NIT-vector or NIT–hAAT cells as target cells. Target cells cultured with unprimed splenocytes were utilized as control cells. The stimulation index (SI) of NIT-1 primed lymphocytes co-cultured with NIT-1 or NIT-vector or NIT–hAAT cells (A).The stimulation index (SI) of NIT-vector primed lymphocytes co-cultured with NIT-1 or NIT-vector or NIT–hAAT cells (B), The stimulation index (SI) of NIT-hAAT primed lymphocytes co-cultured with NIT-1 or NIT-vector or NIT–hAAT cells (C). *P<0.05.