Figure 4. ZFP580 overexpression in H9c2 cells inhibited SI/R-induced apoptosis and caspase-3 activation.

H9C2 cells were transfected with lentiviral vectors expressing full-length ZFP580 (lenti-ZFP580), siRNA directed against ZFP580 (lenti-siRNA) or negative control (lenti-NC) for 72 hours, then underwent SI/R. (A–C): Western blot analysis revealed significant effects of ZFP580 overexpression on cleavage of caspase-3. H9c2 cells (1×10⁶) were collected and stained with Annexin V-PE/7-AAD, and quantified by flow cytometry. (D) Dot plots showing Annexin-V-PE/7-AAD staining of H9c2 cells examined by flow cytometry. (E) Percentage of apoptotic cells as measured by Annexin-V staining in each of the indicated treatments. Three experiments were performed for each group and each experiment was replicated twice. Values are presented as mean ± SEM. *p<0.05 vs. NC.