Table 3. Pseudouridine incorporation measured with various hPus1p enzymes.

	RNA substrates
Enzymea	tRNAAsp	H7 SRA full length	H7 SRA truncation 1	H7 SRA truncation 2	H7 SRA truncation 3	H7 SRA truncation 4
FL hPus1p	0.46 (0.01)	0.02 (<0.01)	0.12 (<0.01)	0.01 (<0.01)	0.05 (<0.01)	0.02 (<0.01)
ΔhPus1p	0.08 (0.01)	0.00 (<0.01)	0.10 (0.01)	0.01 (<0.01)	0.01 (0.02)	0.02 (0.02)
D146A ΔhPus1p	0.01 (<0.01)	0.00 (<0.01)	0.00 (<0.01)	ND	0.00 (<0.01)	ND

^aThe activities are in moles ψ/moles RNA with the mean of three separate assays and standard deviation (SD). With all of the substrates the maximum activity should be ∼1.0 moles ψ/moles RNA. Background levels of activity seen with Lac (β-galactosidase) have been subtracted. ND indicates that the combination of D146A ΔhPus1p D146A and the corresponding RNA substrate was not determined.