Figure 3. Toca-1 and IcsB reciprocally co-precipitate.
Reciprocal co-precipitation of His-Toca-1105–477 and MBP-IcsB from soluble fractions of E. coli BL21 DE3 that co-express the two proteins or the relevant controls, using amylose (for MBP, panel A) or cobalt (for His, panel B) resin. Western blot analysis. The apparent degradation observed for the Toca-1 construct was independent of IcsB. Apparent molecular weights in kilodaltons are indicated to the left of the blots. P, pellet; S, supernatant.