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. 2014 Apr 10;9(4):e94653. doi: 10.1371/journal.pone.0094653

Figure 7. The pro-autophagy activity of Toca-1 observed during infection with Salmonella Typhimurium is absent in the setting of S. flexneri infection.

Figure 7

(A) Levels of Toca-1 in stable Toca-1 KD or control (Toca-1+/+) cells by western blot. 1x and 2x, relative loading. (B) LC3 recruitment around S. Typhimurium or WT or icsA S. flexneri at 40 min. of infection of Toca-1+/+ or Toca-1 KD cells. For each strain, the values are normalized to LC3 recruitment in the presence of Toca-1. (C) LC3 recruitment around icsA S. flexneri at 40 min. of infection of Toca-1 KD cells complemented with Myc-tagged shRNA-resistant Toca-1 [pMyc-Toca-1 (RNAi-resist)] or Myc alone. Data represent the mean ± S.D. of three independent experiments. *P<0.05.