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(A) PCR verification of expected target length using LAMP outer primers F3 and B3. Lane M, 50 bp DNA ladder (Molecular weight marker XIII, Roche); lanes L1 and L2: Loa loa DNA; lane N, negative control (no DNA template). (B) Detection limit of PCR. Lane M, 50 bp DNA ladder (Molecular weight marker XIII, Roche); lane L: Loa loa DNA (5 ng); lanes 10−1–10−6: 10-fold serially dilutions of L. loa DNA; lane N, negative control (no DNA template).
