Figure 5. Sensitivity assessment of the LAMP assay for Loa loa performed with a heating block using simulated human blood samples spiked with serial dilutions of genomic DNA by the addition of SYBR Green I (A) or by visualization on agarose gel stained with ethidium bromide (B).
Lane L1: genomic DNA from Loa loa (5 ng) as positive control; lane L2: human blood sample spiked with 5 ng of genomic DNA from Loa loa; lanes 10−1–10−12: human blood samples spiked with 10-fold serially dilutions; lane N: negative control (no DNA template). Lane M: 50 bp DNA ladder (Molecular weight marker XIII, Roche).