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. 2014 Apr 10;9(4):e94604. doi: 10.1371/journal.pone.0094604

Figure 7. ChIP assay analyses for the enrichment of acetylated H3K9 on the VEGF promoter.

Figure 7

For quantitative ChIP analysis, chromatin was extracted from hearts of each group and precipitated with antibodies against H3K9ace. Quantitative PCR was performed to amplify VEGF promoter regions. Results were normalized with respect to input and nonspecific IgG results by using formula [2-(△ Ct) specific antibody/2-(△ Ct) nonspecific IgG], where △Cp is the Cp (immunoprecipitated DNA)- Cp(input) and Cp is the cycle where the threshold is crossed. The bps on the top of each figure shows positions of upstream (+) or downstream (−) of transcription start site on VEGF promoter region (TSS). Data were showed as means ± SD, n = 4, *P<0.05, **P<0.01 vs. Control, # P<0.01vs MI.