Figure 2. Residues in the linker region of FtsZ are important for degradation by ClpXP.

A. Alignment of FtsZ residues 349 through 358 from the linker domain with the C-terminal ClpX recognition region of Mu repressor protein from phage Mu. B. Linear schematic diagram of FtsZ showing regions of the linker that were tested by triple alanine scanning mutagenesis of wild type FtsZ and truncated FtsZ(Δ_375-383). C. Comparison of rates of degradation of wild type and mutant FtsZ, proteins in the presence and absence of GTP from in vitro degradation reactions containing 10 µM wild type or mutant fluorescent FtsZ and 1 µM ClpXP. Data from 3 replicates are presented as mean ± SEM.