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. 2014 Apr 10;9(4):e94964. doi: 10.1371/journal.pone.0094964

Figure 3. FtsZ mutant proteins with C-terminal mutations hydrolyze GTP and assemble into polymers.

Figure 3

(A) Rates of GTP hydrolysis were measured in reactions containing FtsZ wild type or mutant (5 µM) and GTP (1 mM) as described in Experimental Procedures. Data from 3 replicates are presented as mean ± SEM. (B) GTP-dependent assembly of FtsZ wild type and mutant proteins (8 µM) was monitored by 90° light scattering as described in Experimental Procedures. A baseline was collected for 2 min, then GTP was added when indicated, to stimulate polymerization. Light scattering was measured for 25 min. Data shown is representative of 3 replicates.