Figure 6. MinC competes with ClpXP for FtsZ in vitro.

A. MinC was included in degradation reactions containing FtsZ with GTP and ClpXP (0.5 µM). B. FtsZ (5 µM; 125 pmol/reaction) was first preincubated with GTP (2 mM) and then incubated with MinC (2 µM), ClpXP (0.5 µM) and ATP, as indicated. FtsZ polymer disruption was monitored by measuring the amount of fluorescent FtsZ present in high-speed centrifugation pellets. In A and B data from 3 replicates are presented as mean ± SEM.