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. 2014 Apr 10;9(4):e94999. doi: 10.1371/journal.pone.0094999

Figure 2. The C-terminal region of IKKε contains two functional domains required for IFNβ promoter activity.

Figure 2

(A) Schematic structure of IKKε and C-terminal truncated mutants. The putative functional domains (641–657 and 686–705) are shown. (B) 293T cells were transfected with the IFNβ promoter-luciferase reporter along with the FLAG-tagged full length IKKε (1-716), kinase defective mutant (K38A) or C-terminal deletion mutants presented in (A). Cells were lysed at 24 h post-transfection and luciferase activities were quantified by normalization with renilla luciferase activity. The values represent the average of three samples +/− SD. Cell lysates were also subjected to SDS-PAGE and Western-blotted with the antibodies indicated on the left. (C) 293T cells were transfected with the IFNβ promoter-luciferase reporter along with plasmid for wt or mutant forms of TKB1 and IKKε. Luciferase activities were measured as shown in (B).