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. 2014 Apr 10;9(4):e94999. doi: 10.1371/journal.pone.0094999

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© 2014 Nakatsu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) V5-tagged IKKε and FLAG-tagged wt or the indicated C-terminal truncated mutants of IKKε were co-transfected into 293T cells. Cells were lysed 24 h post-transfection. Lysates were immunoprecipitated with FLAG-agarose and V5-tagged IKKε were detected with an anti-V5 antibody (top panel). V5 and FLAG-tagged IKKε in whole cell lysate (WCE) were also detected as indicated on the left. (B) V5-tagged IKKε and FLAG-tagged wt or IKKε mutants with a single amino acid substitution, as indicated at the top, were co-transfected into 293T cells. Immunoprecipitation was performed as in (A).