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. 2014 Apr 10;9(4):e94721. doi: 10.1371/journal.pone.0094721

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© 2014 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Control (A–C) and 10 µM cadmium-treated (D–F) pollen tubes were labeled with ER-Tracker Green. Differential interference contrast images (A, D) and corresponding fluorescent images (B, E) were obtained using a Zeiss 5 live confocal microscope. In total, 11 control tubes and 20 cadmium-treated tubes were examined. No obvious individual differences were observed within control or cadmium-treated tubes, respectively. Images C and F represents magnified views of the tip regions in images B and E, respectively. Images show that cadmium reduced the amount of ER and number of Golgi apparatus, and induced vacuole-like organelles. Bars = 10 µm.