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. 2014 Apr 10;9(4):e94855. doi: 10.1371/journal.pone.0094855

Figure 4. miR-143 directly targets Syn-1.

Figure 4

(A) Association of miR-143 and Syn-1 with Ago2. Cellular lysates from sk-mel-5 cells transfected with or without miR-143 mimics were used for RNA immunoprecipitation with Ago2 antibody. Detection of Syn-1 (B) and miR-143 (C) in the same RISC complex using real-time PCR. (D)The repression of luciferase activity by Syn-1 3′UTR was dependent on miR-143. Mutated Syn-1 3′UTR abrogated miR-143 mediated repression luciferase activity (**P<0.01, Figure is representative of 3 experiments with similar results.)