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Michaelis–Menten assays for GOX and HRP in the PEG-rich phase (open circles) and citrate-rich phase (closed circles). (A) Effect of glucose concentration on GOX rate, measured at 0.05 U/mL GOX (2.1 nM). Effect of substrate concentration on HRP rate for (B) peroxide and (C) Amplex Red. HRP concentrations were 0.005 U/mL (0.45 nM) for PEG-rich phase experiments and 0.0005 U/mL (0.045 nM) for the citrate-rich phase.
