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. 2014 Feb 11;118(9):2506–2517. doi: 10.1021/jp501126v

Table 1. Michaelis–Menten Constants of GOX and HRP within PEG:Citrate ATPS.

	K_M (μM)	V_max (μmol/min/mg)	k_cat (s^–1)
GOX (Glucose)
PEG-rich	3400 ± 400	73 ± 2^a	194 ± 5
citrate-rich	5400 ± 200	66.9 ± 0.6^a	178 ± 2
HRP (H₂O₂)
PEG-rich	5 ± 1	28 ± 2^b	20 ± 1
citrate-rich	150 ± 20	2600 ± 200^c	1900 ± 100
HRP (Amplex Red)
PEG-rich	n.a.^d	n.a.^b	n.a.
citrate-rich	60 ± 10	2300 ± 100^c	1700 ± 100

^a

V_max for enzyme concentrations of 0.05 U/mL.

^b

V_max for enzyme concentrations of 0.005 U/mL.

^c

V_max for enzyme concentrations of 0.0005 U/mL.

^d

Not applicable. Rate increased linearly to the limit of substrate solubility.