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. 2014 Feb 18;57(4):1208–1224. doi: 10.1021/jm401552y

Figure 3.

Figure 3

Hsp70–HOP dissociation and HER2 degradation by 1a and 3a. SKBr3 cells were treated for 24 h with vehicle or indicated concentrations of 1a or 3a. Hsp70–HOP dissociation: Upon cell lysing, Hsp70 complexes were isolated with an anti-Hsp70 antibody (IP BB70) and analyzed by Western blotting (WB). Specificity of binding was tested with a control IgG. HER2 degradation: Proteins in the lysate were analyzed by immonoblotting with an anti-HER2 antibody. β-Actin was used to control for equal loading. Gels were quantified by densitometry, values normalized to the control (vehicle only treated cells), and data graphed against the Hsp70 inhibitor concentration. Error bars represent the SD of the mean (n = 3).