Following determination
of the current elution order range (A),
target peptides (B) sharing a similar elution order value are selected
(C, rectangles represent individual peptides). Peptide targets within
the elution order range are filtered based on when they were last
sampled for MS/MS (D), leaving only targets that have been waiting
the longest (e.g., > 5 s, highlighted rectangles). Those filtered
peptides are then immediately sampled by MS/MS, regardless of MS1 detection (D). Unfilled MS/MS events are automatically filled
with m/z features picked by the
intensity-based DDA algorithm using normal sampling parameters (e.g.,
dynamic exclusion, intensity threshold, charge state exclusion, etc.).