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(A) Four C57Bl/6 mice were sacrificed at 10 weeks of age, and eight organs were harvested from each mouse. (B) Peptides resulting from a tryptic digestion of lysates from each organism were labeled with TMT 8-plex tags in a randomized order. (C) 165 min nano-LC–MS/MS experiments using DDA top-15 method identified only 3969 peptides in all four mice. (D) A subset of 1500 peptide targets was selected from peptides detected in only two or three of all four mice.
