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. 2014 Jan 22;137(3):819–833. doi: 10.1093/brain/awt355

Figure 2.

Figure 2

Glucan encapsulated small interfering RNA particles (GeRPs) can effectively knock-down total HTT in primary human immune cells. (A) GeRPs deliver small interfering RNA (siRNA) efficiently when phagocytosed by myeloid cells, as shown in primary human monocytes after 12 h incubation in culture (GeRPs = green; DAPI = blue). (B) Ninety per cent of macrophages take up GeRPs when incubated at 1:10 cell: particle ratio for 12 h as quantified by flow cytometry. Data shown as mean [n = 2 for controls and n = 3 for Huntington’s disease (HD)] ± SEM. (C) Total HTT RNA measured by quantitative PCR and protein levels measured by TR-FRET were reduced by 70% and 50%, respectively, in macrophages treated for 3 days with GeRPs containing anti-HTT small interfering RNA. Data shown as mean HTT levels (each combining two independent experiments, n = individual biological repeats) ± SEM. Data are normalized to the scrambled small interfering RNA treated condition for each genotype.