Figure 3.

Knock-down of total HTT reverses the hyper-reactive cytokine production by Huntington’s disease macrophages. Huntington’s disease (HD) and control macrophages were treated with either anti-HTT or scrambled small interfering RNA (siRNA) for 3 days, before the cells were stimulated with 10 ng/ml IFNγ and 2 µg/ml lipopolysaccharide for 24 h. Measuring cytokine production with multiplex ELISA assays showed that lowering HTT levels reduces IL-6, IL-8 and TNFα levels after stimulation. Data shown as mean concentrations (n = 9 for controls and n = 8 for Huntington’s disease, combined from three independent experiments, n = individual biological repeats) ± SEM, two-way ANOVA with Bonferroni post-tests. *P < 0.05; **P < 0.01, ***P < 0.001.