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L5 SNL induces GPRC5B downregulation in spinal neurons, but not in microglia. (A, B) GPRC5B colocalizes with NeuN (a neuron marker), but not with OX42 (a microglia marker), in both the ipsilateral and contralateral dorsal horn. Double-labeled cells appear yellow in the merged images. Scale bar, 100 µm. (C, D) Double staining of GPRC5B and GFAP (an astrocyte marker) or CNPase (an oligodendrocyte marker) showed no colocalization. Scale bar, 100 µm.
